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Article Dans Une Revue EMBO Reports Année : 2009

The SPX domain of the yeast low-affinity phosphate transporter Pho90 regulates transport activity

Résumé

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission. Yeast has two phosphate-uptake systems that complement each other: the high-affinity transporters (Pho84 and Pho89) are active under phosphate starvation, whereas Pho87 and Pho90 are low-affinity transporters that function when phosphate is abundant. Here, we report new regulatory functions of the amino-terminal SPX domain of Pho87 and Pho90. By studying truncated versions of Pho87 and Pho90, we show that the SPX domain limits the phosphate-uptake velocity, suppresses phosphate efflux and affects the regulation of the phosphate signal transduction pathway. Furthermore, split-ubiquitin assays and co-immuno-precipitation suggest that the SPX domain of both Pho90 and Pho87 interacts physically with the regulatory protein Spl2. This work suggests that the SPX domain inhibits low-affinity phosphate transport through a physical interaction with Spl2.
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hal-02354942 , version 1 (08-11-2019)

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Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales

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Hans Caspar Hürlimann, Benoit Pinson, Martha Stadler-Waibel, Samuel Zeeman, Florian M Freimoser. The SPX domain of the yeast low-affinity phosphate transporter Pho90 regulates transport activity. EMBO Reports, 2009, 10 (9), pp.1003-1008. ⟨10.1038/embor.2009.105⟩. ⟨hal-02354942⟩

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