The PDZ Protein Mupp1 Promotes G i Coupling and Signaling of the Mt 1 Melatonin Receptor - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2008

The PDZ Protein Mupp1 Promotes G i Coupling and Signaling of the Mt 1 Melatonin Receptor

Résumé

Intracellular signaling events are often organized around PDZ (PSD-95/Drosophila Disc large/ZO-1 homology) domain-containing scaffolding proteins. The ubiquitously expressed multi-PDZ protein MUPP1, which is composed of 13 PDZ domains, has been shown to interact with multiple viral and cellular proteins and to play important roles in receptor targeting and trafficking. In this study, we show that MUPP1 binds to the G protein-coupled MT 1 melatonin receptor and directly regulates its G i-dependent signal transduction. Structural determinants involved in this interaction are the PDZ10 domain of MUPP1 and the valine of the canonical class III PDZ domain binding motif DSV of the MT 1 carboxyl terminus. This high affinity interaction (K d ϳ 4 nM), which is independent of MT 1 activation, occurs in the ovine pars tuberalis of the pituitary expressing both proteins endogenously. Although the disruption of the MT 1 /MUPP1 interaction has no effect on the subcel-lular localization, trafficking, or degradation of MT 1 , it destabilizes the interaction between MT 1 and G i and abolishes G i-mediated signaling of MT 1. Our findings highlight a previously unappreciated role of PDZ proteins in promoting G protein coupling to receptors.

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hal-02348057 , version 1 (31-05-2020)

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Jean-Luc Guillaume, Avais M Daulat, Pascal Maurice, Angélique Levoye, Martine Migaud, et al.. The PDZ Protein Mupp1 Promotes G i Coupling and Signaling of the Mt 1 Melatonin Receptor. Journal of Biological Chemistry, 2008, 283 (24), pp.16762-16771. ⟨10.1074/jbc.M802069200⟩. ⟨hal-02348057⟩
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