Structural Insights into the Forward and Reverse Enzymatic Reactions in Human Adenine Phosphoribosyltransferase
Résumé
Highlights d Human APRT catalyzes the transformation of adenine into AMP and vice versa d Complexes with substrates in both directions of the reaction highlight key residues d The catalytic flexible loop dynamic is revealed by an in crystallo activity d Tyr105 is essential for cell growth by facilitating the forward reaction In Brief APRT is a key enzyme in the purine salvage pathway in prokaryotes and eukaryotes. Huyet et al., by using in vitro, cellular, and in crystallo enzymatic analyses, reveal that a hydroxyl group in a conserved tyrosine controls the protein dynamics and the catalytic efficiencies of the forward and reverse reactions.
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers éditeurs autorisés sur une archive ouverte
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