Histone mark recognition controls nucleosome translocation via a kinetic proofreading mechanism: Confronting theory and high-throughput experiments - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Physical Review E Année : 2019

Histone mark recognition controls nucleosome translocation via a kinetic proofreading mechanism: Confronting theory and high-throughput experiments

Résumé

Chromatin remodelers are multi-domain enzymatic motor complexes that displace nucleosomes along DNA and hence 'remodel chromatin structure', i.e., they dynamically reorganize nucleosome positions in both gene activation and gene repression. Recently, experimental insights from structural biology methods and remodeling assays have substantially advanced the understanding of these key chromatin components. Here we confront the kinetic proofreading scenario of chromatin remodeling, which proposes a mechanical link between histone residue modifications and the ATP-dependent action of remodelers, with recent experiments. We show that recent high-throughput data on nucleosome libraries assayed with remodelers from the ISWI family are in accord with our earlier predictions of the kinetic proofreading scenario. We make suggestions for new experimentally verifiable predictions of the kinetic proofreading scenarios for remodelers from other families.
Fichier principal
Vignette du fichier
pre-rc-proofreading.pdf (1.19 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02344794 , version 1 (04-11-2019)

Identifiants

Citer

Ralf Blossey, Helmut Schiessel. Histone mark recognition controls nucleosome translocation via a kinetic proofreading mechanism: Confronting theory and high-throughput experiments. Physical Review E , 2019, 99 (6), ⟨10.1103/PhysRevE.99.060401⟩. ⟨hal-02344794⟩

Collections

CNRS UNIV-LILLE
13 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More