The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-kappaB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Oncology Année : 2002

The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-kappaB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells.

Bertrand Liagre
Raphael E Duval
Arnaud Bianchi
Karim Bordji
Jean-Louis Beneytout
  • Fonction : Auteur
  • PersonId : 901632

Résumé

Non-steroidal anti-inflammatory drugs (NSAIDs) have been shown to produce an anti-proliferative and pro-apoptotic effect on different types of cancer cell lines. Previously, we demonstrated that high dose of NS-398 (100 microM), a selective cyclooxygenase-2 inhibitor, induced a cell cycle slowing or arrest and, in contrast to low dose (10 microM), a marked decrease in apoptosis in human 1547 osteosarcoma cells. In this study, we investigated particularly the effect of 100 microM NS-398 on p53 and p21 expression, caspase activities and nuclear factor-kappaB (NF-kappaB). We found a correlation between p53, p21 mRNA expression and NF-kappaB activation and, we observed an induction of heat shock protein 70 expression with a large decrease in caspase-3 activity after 100 microM NS-398 treatment. Moreover, the inhibition of apoptosis was correlated with an increase in bcl-2/bax ratio. Our new findings confirm the novel anti-apoptotic property of NS-398 at 100 microM, as we previously found, which contrasts to the described NS-398 pro-apoptotic effect on other cancer cell lines.
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Dates et versions

hal-02340688 , version 1 (30-10-2019)

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Citer

Sandra Moalic-Juge, Bertrand Liagre, Raphael E Duval, Cécile Corbière, Arnaud Bianchi, et al.. The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-kappaB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells.. International Journal of Oncology, 2002, 20 (6), pp.1255-62. ⟨10.3892/ijo.20.6.1255⟩. ⟨hal-02340688⟩

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