Promyelocytic leukemia-nuclear body formation is an early event leading to retinoic acid-induced differentiation of neuroblastoma cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Neurochemistry Année : 2007

Promyelocytic leukemia-nuclear body formation is an early event leading to retinoic acid-induced differentiation of neuroblastoma cells

A. Delaune
F. Benjelloun
  • Fonction : Auteur
J. Vannier
  • Fonction : Auteur
C. Ripoll
M. Vasse
  • Fonction : Auteur

Résumé

Neuroblastoma is one of the most common cancers in children. Neuroblastoma differentiation is linked to the presence of the promyelocytic leukemia (PML) protein. Retinoic acid, a powerful differentiation-inducer in vitro, is a potent agent for the treatment of neuroblastoma. Using two different human neuroblastoma cell lines, SH-SY5Y and LA-N-5, we show here that PML protein leads to the formation of nuclear bodies (PML-NB) after only 1 h of retinoic acid treatment and that this formation is mediated by the extracellular signal-regulated kinase (ERK) pathway. Inhibition of protein kinase C also leads to formation of PML-NB via the ERK pathway. Both sumoylation and phosphorylation of PML in an ERK-dependent pathway are also required for formation of PML-NB. Finally, we show that PML-NB formation in neuroblastoma cells is associated with neurite outgrowth. These results support the proposal that the formation of PML-NB is correlated with the differentiation of neuroblastoma cells.

Dates et versions

hal-02340675 , version 1 (30-10-2019)

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Citer

A. Delaune, C. Corbière, F. Benjelloun, E. Legrand, J. Vannier, et al.. Promyelocytic leukemia-nuclear body formation is an early event leading to retinoic acid-induced differentiation of neuroblastoma cells. Journal of Neurochemistry, 2007, 104 (1), pp.071106220615005-???. ⟨10.1111/j.1471-4159.2007.05019.x⟩. ⟨hal-02340675⟩
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