Enzymatic Production and Enzymatic-Mass Spectrometric Fingerprinting Analysis of Chitosan Polymers with Different Nonrandom Patterns of Acetylation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2019

Enzymatic Production and Enzymatic-Mass Spectrometric Fingerprinting Analysis of Chitosan Polymers with Different Nonrandom Patterns of Acetylation

Raphaël Vibert
  • Fonction : Auteur
Laurent Duclaux
Laurence Reinert
Jasper Wattjes
  • Fonction : Auteur
Anna Niehues
  • Fonction : Auteur
Stefan Cord-Landwehr
  • Fonction : Auteur
Janina Hossbach
  • Fonction : Auteur
Laurent David
Thierry Delair
  • Fonction : Auteur
  • PersonId : 863899
Bruno Moerschbacher
  • Fonction : Auteur

Résumé

Chitosans, a family of ß-(1,4)-linked, partially N-acetylated polyglucosamines, are considered to be among the most versatile and most promising functional biopolymers. Chemical analysis and bioactivity studies revealed that the functionalities of chitosans strongly depend on the polymers’ degree of polymerization and fraction of acetylation. More recently, the pattern of acetylation (PA) has been proposed as another important parameter to influence functionalities of chitosans. We therefore carried out studies on the acetylation pattern of chitosan polymers produced by three recombinant fungal chitin deacetylases (CDAs) originating from different species, namely, Podospora anserina, Puccinia graminis f. sp. tritici, and Pestalotiopsis sp. We analyzed the chitosans by 1H NMR, 13C NMR, and SEC-MALS and established new methods for PA analysis based on enzymatic mass spectrometric fingerprinting and in silico simulations. Our studies strongly indicate that the different CDAs indeed produce chitosans with different PA. Finally, Zimm plot analysis revealed that enzymatically treated polymers differ with respect to their second virial coefficient and radius of gyration indicating an influence of PA on polymer–solvent interactions.
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Dates et versions

hal-02335453 , version 1 (28-10-2019)

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Imadeddine Lakehal, Alexandra Montembault, Arnaud Perrier, Raphaël Vibert, Laurent Duclaux, et al.. Enzymatic Production and Enzymatic-Mass Spectrometric Fingerprinting Analysis of Chitosan Polymers with Different Nonrandom Patterns of Acetylation. Journal of the American Chemical Society, 2019, 141 (7), pp.3137-3145. ⟨10.1021/jacs.8b12561⟩. ⟨hal-02335453⟩
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