Stem Cell Reports Resource Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
Renata Santos
(1)
,
Krishna C Vadodaria
,
Baptiste N Jaeger
,
Arianna Mei
,
Sabrina Lefcochilos-Fogelquist
,
Ana P.D. Mendes
,
Galina Erikson
,
Maxim Shokhirev
,
Lynne Randolph-Moore
,
Callie Fredlender
,
Sonia Dave
,
Ruth Oefner
,
Conor Fitzpatrick
,
Monique Pena
,
Jerika J Barron
,
Manching Ku
,
Ahmet M Denli
,
Bilal E Kerman
,
Patrick Charnay
(2)
,
John R. Kelsoe
(3)
,
Maria C Marchetto
,
Fred H Gage
(4)
Renata Santos
- Fonction : Auteur
- PersonId : 179805
- IdHAL : renata-santos
- ORCID : 0000-0002-3085-5128
Krishna C Vadodaria
- Fonction : Auteur
Baptiste N Jaeger
- Fonction : Auteur
Arianna Mei
- Fonction : Auteur
Sabrina Lefcochilos-Fogelquist
- Fonction : Auteur
Ana P.D. Mendes
- Fonction : Auteur
Galina Erikson
- Fonction : Auteur
Maxim Shokhirev
- Fonction : Auteur
Lynne Randolph-Moore
- Fonction : Auteur
Callie Fredlender
- Fonction : Auteur
Sonia Dave
- Fonction : Auteur
Ruth Oefner
- Fonction : Auteur
Conor Fitzpatrick
- Fonction : Auteur
Monique Pena
- Fonction : Auteur
Jerika J Barron
- Fonction : Auteur
Manching Ku
- Fonction : Auteur
Ahmet M Denli
- Fonction : Auteur
Bilal E Kerman
- Fonction : Auteur
Maria C Marchetto
- Fonction : Auteur
Résumé
Astrocyte dysfunction and neuroinflammation are detrimental features in multiple pathologies of the CNS. Therefore, the development of methods that produce functional human astrocytes represents an advance in the study of neurological diseases. Here we report an efficient method for inflammation-responsive astrocyte generation from induced pluripotent stem cells (iPSCs) and embryonic stem cells. This protocol uses an intermediate glial progenitor stage and generates functional astrocytes that show levels of glutamate uptake and calcium activation comparable with those observed in human primary astrocytes. Stimulation of stem cell-derived astrocytes with interleukin-1b or tumor necrosis factor a elicits a strong and rapid pro-inflammatory response. RNA-sequencing transcriptome profiling confirmed that similar gene expression changes occurred in iPSC-derived and primary astrocytes upon stimulation with interleukin-1b. This protocol represents an important tool for modeling in-a-dish neuro-logical diseases with an inflammatory component, allowing for the investigation of the role of diseased astrocytes in neuronal degeneration.
Domaines
Sciences du Vivant [q-bio]Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Stem Cell Reports Resource Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
|
Résumé |
en
Astrocyte dysfunction and neuroinflammation are detrimental features in multiple pathologies of the CNS. Therefore, the development of methods that produce functional human astrocytes represents an advance in the study of neurological diseases. Here we report an efficient method for inflammation-responsive astrocyte generation from induced pluripotent stem cells (iPSCs) and embryonic stem cells. This protocol uses an intermediate glial progenitor stage and generates functional astrocytes that show levels of glutamate uptake and calcium activation comparable with those observed in human primary astrocytes. Stimulation of stem cell-derived astrocytes with interleukin-1b or tumor necrosis factor a elicits a strong and rapid pro-inflammatory response. RNA-sequencing transcriptome profiling confirmed that similar gene expression changes occurred in iPSC-derived and primary astrocytes upon stimulation with interleukin-1b. This protocol represents an important tool for modeling in-a-dish neuro-logical diseases with an inflammatory component, allowing for the investigation of the role of diseased astrocytes in neuronal degeneration.
|
Auteur(s) |
Renata Santos
1
, Krishna C Vadodaria
, Baptiste N Jaeger
, Arianna Mei
, Sabrina Lefcochilos-Fogelquist
, Ana P.D. Mendes
, Galina Erikson
, Maxim Shokhirev
, Lynne Randolph-Moore
, Callie Fredlender
, Sonia Dave
, Ruth Oefner
, Conor Fitzpatrick
, Monique Pena
, Jerika J Barron
, Manching Ku
, Ahmet M Denli
, Bilal E Kerman
, Patrick Charnay
2
, John R. Kelsoe
3
, Maria C Marchetto
, Fred H Gage
4
1
IPNP - U1266 Inserm - Paris Descartes -
Institut de psychiatrie et neurosciences de Paris
( 553485 )
- 102-108 Rue de la Santé
75014 PARIS
- France
2
IBENS -
Institut de biologie de l'ENS Paris
( 106195 )
- 46, Rue d’Ulm
75005 Paris
- France
3
Department of Psychiatry
( 172314 )
- La Jolla, California
- États-Unis
4
The Salk Institute for Biological Studies
( 523654 )
- 10010 North Torrey Pines Road, La Jolla, CA, 92037
- États-Unis
|
Licence |
Paternité - Pas d'utilisation commerciale - Pas de modification
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2017-06
|
Volume |
8
|
Numéro |
6
|
Page/Identifiant |
1757-1769
|
Domaine(s) |
|
DOI | 10.1016/j.stemcr.2017.05.011 |
Origine :
Publication financée par une institution
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