Recombinant Antibodies against Mycolactone - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Toxins Année : 2019

Recombinant Antibodies against Mycolactone

Résumé

In the past, it has proved challenging to generate antibodies against mycolactone, the primary lipidic toxin A of Mycobacterium ulcerans causing Buruli ulcer, due to its immunosuppressive properties. Here we show that in vitro display, comprising both phage and yeast display, can be used to select antibodies recognizing mycolactone from a large human naïve phage antibody library. Ten different antibodies were isolated, and hundreds more identified by next generation sequencing. These results indicate the value of in vitro display methods to generate antibodies against difficult antigenic targets such as toxins, which cannot be used for immunization unless inactivated by structural modification. The possibility to easily generate anti-mycolactone antibodies is an exciting prospect for the development of rapid and simple diagnostic/detection methods.

Domaines

Immunologie
Fichier principal
Vignette du fichier
toxins-11-00346-v2.pdf (1.24 Mo) Télécharger le fichier
image.png (102.09 Ko) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-02323359 , version 1 (18-12-2019)

Licence

Paternité

Identifiants

Citer

Leslie Naranjo, Fortunato Ferrara, Nicolas Blanchard, Caroline Demangel, Sara D’angelo, et al.. Recombinant Antibodies against Mycolactone. Toxins, 2019, 11 (6), pp.346. ⟨10.3390/toxins11060346⟩. ⟨hal-02323359⟩
52 Consultations
29 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More