Impact of Hemoglobin S Trait on Cell Surface Antibody Recognition of Plasmodium falciparum-Infected Erythrocytes in Pregnancy-Associated Malaria - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Open Forum Infectious Diseases Année : 2019

Impact of Hemoglobin S Trait on Cell Surface Antibody Recognition of Plasmodium falciparum-Infected Erythrocytes in Pregnancy-Associated Malaria

Margaux Chauvet
  • Fonction : Auteur
Florentin Aussenac
  • Fonction : Auteur
Emeline Brossier
  • Fonction : Auteur
Luc Denoyel
  • Fonction : Auteur
Marion Hanny
  • Fonction : Auteur
Damien Pineau
  • Fonction : Auteur
Benoît Gamain

Résumé

Background. Sickle cell trait (HbAS) confers partial protection against malaria by reducing the adhesion of Plasmodium falciparum-infected erythrocytes to host receptors, but little is known about its potential protection against placental malaria. Methods. Using flow cytometry, we assessed the recognition of HbAA and HbAS VAR2CSA-expressing infected erythrocytes, by plasma from 159 Beninese pregnant women with either HbAA (normal) or HbAS. Using multivariate linear models adjusted for gravidity, parasite infection at delivery, glucose-6-phosphate dehydrogenase deficiency, and α-thalassemia carriage, we observed significantly reduced cell surface antibody binding of HbAS-infected erythrocytes by plasma from HbAS compared with HbAA women (P < 10-3). Results. The difference in cell surface antibody binding was only observed when infected erythrocytes and plasma were associated according to the same hemoglobin genotype. Similar levels of VAR2CSA-specific antibody were measured by enzyme-linked immunosorbent assay in the 2 groups, suggesting that the altered interaction between VAR2CSA and HbAS women's antibodies could reflect abnormal display of VAR2CSA on HbAS erythrocytes. Conclusions. Our data stress the need for assessments of erythrocyte disorders such as the sickle cell trait in a population group when studying immunological responses to P falciparum.
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hal-02323104 , version 1 (01-12-2020)

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Margaux Chauvet, Marilou Tetard, Gilles Cottrell, Florentin Aussenac, Emeline Brossier, et al.. Impact of Hemoglobin S Trait on Cell Surface Antibody Recognition of Plasmodium falciparum-Infected Erythrocytes in Pregnancy-Associated Malaria. Open Forum Infectious Diseases, 2019, 6 (4), ⟨10.1093/ofid/ofz156⟩. ⟨hal-02323104⟩
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