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Article Dans Une Revue Biophysical Journal Année : 2010

Order Parameters of a Transmembrane Helix in a Fluid Bilayer: Case Study of a WALP Peptide

Résumé

A new solid-state NMR-based strategy is established for the precise and efficient analysis of orientation and dynamics of transmembrane peptides in fluid bilayers. For this purpose, several dynamically averaged anisotropic constraints, including 13C and 15N chemical shift anisotropies and 13C-15N dipolar couplings, were determined from two different triple-isotope-labeled WALP23 peptides (2H, 13C, and 15N) and combined with previously published quadrupolar splittings of the same peptide. Chemical shift anisotropy tensor orientations were determined with quantum chemistry. The complete set of experimental constraints was analyzed using a generalized, four-parameter dynamic model of the peptide motion, including tilt and rotation angle and two associated order parameters. A tilt angle of 21° was determined for WALP23 in dimyristoylphosphatidylcholine, which is much larger than the tilt angle of 5.5° previously determined from 2H NMR experiments. This approach provided a realistic value for the tilt angle of WALP23 peptide in the presence of hydrophobic mismatch, and can be applied to any transmembrane helical peptide. The influence of the experimental data set on the solution space is discussed, as are potential sources of error.
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Dates et versions

hal-02322509 , version 1 (11-04-2023)

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Andrea Holt, Léa Rougier, Valérie Réat, Franck Jolibois, Olivier Saurel, et al.. Order Parameters of a Transmembrane Helix in a Fluid Bilayer: Case Study of a WALP Peptide. Biophysical Journal, 2010, 98 (9), pp.1864-1872. ⟨10.1016/j.bpj.2010.01.016⟩. ⟨hal-02322509⟩
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