Impact of biological matrix on inflammatory protein biomarker quantification based on targeted mass spectrometry - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Bioanalysis Année : 2018

Impact of biological matrix on inflammatory protein biomarker quantification based on targeted mass spectrometry

Résumé

BACKGROUND: Serum and plasma are widely used matrices in biological and clinical studies. To improve reliability and consistency of markers quantification, the influence of these matrices on proteins was evaluated by targeted mass spectrometry. RESULTS: 65 proteins were quantified in matched blood samples collected in serum, ethylenediaminetetraacetic acid and heparin plasma tubes from 40 healthy and 10 pathological individuals. Only 52% of the proteins were not impacted by any of the biological matrices tested, and the effects on quantification of proteins affected was matrix and protein dependent. CONCLUSION: Matrix comparisons using mass spectrometry is therefore recommended to assess the relevance of using surrogate matrix, performing biomarker discovery study or evaluating the clinical use of biomarkers in large clinical cohorts.
Fichier non déposé

Dates et versions

hal-02316826 , version 1 (15-10-2019)

Identifiants

Citer

Anne Incamps, Celia Saez-Boiteau, Svenja Lena Tiede, Pauline Rebillard, Janin Schulte, et al.. Impact of biological matrix on inflammatory protein biomarker quantification based on targeted mass spectrometry. Bioanalysis, 2018, 10 (17), pp.1383--1399. ⟨10.4155/bio-2018-0149⟩. ⟨hal-02316826⟩
39 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More