As widespread, continuous instrumental Earth surface air temperature records are available only for the last hundred fifty years, indirect reconstructions of past temperatures are obtained by analyzing "proxies". Fluid inclusions (FIs) present in virtually all rock minerals including exogenous rocks are routinely used to constrain formation temperature of crystals. The method relies on the presence of a vapour bubble in the FI. However, measurements are sometimes biased by surface tension effects. They are even impossible when the bubble is absent (monophasic FI) for kinetic or thermodynamic reasons. These limitations are common for surface or subsurface rocks. Here we use FIs in hydrothermal or geodic quartz crystals to demonstrate the potential of Brillouin spectroscopy in determining the formation temperature of monophasic FIs without the need for a bubble. Hence, this novel method offers a promising way to overcome the above limitations.