Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Skeletal Muscle Année : 2019

Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle

Thibaut Desgeorges
  • Fonction : Auteur
Sophie Liot
  • Fonction : Auteur
Solène Lyon
  • Fonction : Auteur
Jessica Bouviere
  • Fonction : Auteur
Alix Kemmel
  • Fonction : Auteur
Aurélie Trignol
  • Fonction : Auteur
Bruno Chapuis
  • Fonction : Auteur
Julien Gondin
Rémi Mounier
Benedicte Chazaud
  • Fonction : Auteur
Gaetan Juban
  • Fonction : Auteur

Résumé

Adult skeletal muscle is capable of complete regeneration after an acute injury. The main parameter studied to assess muscle regeneration efficacy is the cross-sectional area (CSA) of the myofibers as myofiber size correlates with muscle force. CSA analysis can be time-consuming and may trigger variability in the results when performed manually. This is why programs were developed to completely automate the analysis of the CSA, such as SMASH, MyoVision, or MuscleJ softwares. Although these softwares are efficient to measure CSA on normal or hypertrophic/atrophic muscle, they fail to efficiently measure CSA on regenerating muscles. We developed Open-CSAM, an ImageJ macro, to perform a high throughput semi-automated analysis of CSA on skeletal muscle from various experimental conditions. The macro allows the experimenter to adjust the analysis and correct the mistakes done by the automation, which is not possible with fully automated programs. We showed that Open-CSAM was more accurate to measure CSA in regenerating and dystrophic muscles as compared with SMASH, MyoVision, and MuscleJ softwares and that the inter-experimenter variability was negligible. We also showed that, to obtain a representative CSA measurement, it was necessary to analyze the whole muscle section and not randomly selected pictures, a process that was easily and accurately be performed using Open-CSAM. To conclude, we show here an easy and experimenter-controlled tool to measure CSA in muscles from any experimental condition, including regenerating muscle.
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Dates et versions

hal-02307736 , version 1 (15-02-2023)

Identifiants

Citer

Thibaut Desgeorges, Sophie Liot, Solène Lyon, Jessica Bouviere, Alix Kemmel, et al.. Open-CSAM, a new tool for semi-automated analysis of myofiber cross-sectional area in regenerating adult skeletal muscle. Skeletal Muscle, 2019, 9 (2), pp.1-12. ⟨10.1186/s13395-018-0186-6⟩. ⟨hal-02307736⟩
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