Glycine transporter dimers: evidence for occurrence in the plasma membrane. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2008

Glycine transporter dimers: evidence for occurrence in the plasma membrane.

Résumé

Different Na(+)/Cl(-)-dependent neurotransmitter transporters of the SLC6a family have been shown to form dimers or oligomers in both intracellular compartments and at the cell surface. In contrast, the glycine transporters (GlyTs) GlyT1 and -2 have been reported to exist as monomers in the plasma membrane based on hydrodynamic and native gel electrophoretic studies. Here, we used cysteine substitution and oxidative cross-linking to show that of GlyT1 and GlyT2 also form dimeric complexes within the plasma membrane. GlyT oligomerization at the cell surface was confirmed for both GlyT1 and GlyT2 by fluorescence resonance energy transfer microscopy. Endoglycosidase treatment and surface biotinylation further revealed that complex-glycosylated GlyTs form dimers located at the cell surface. Furthermore, substitution of tryptophan 469 of GlyT2 by an arginine generated a transporter deficient in dimerization that was retained intracellulary. Based on these results and GlyT structures modeled by using the crystal structure of the bacterial homolog LeuT(Aa), as a template, residues located within the extracellular loop 3 and at the beginning of transmembrane domain 6 are proposed to contribute to the dimerization interface of GlyTs.
Fichier principal
Vignette du fichier
J. Biol. Chem-Bartholomäus-2008.pdf (1.25 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02306874 , version 1 (07-10-2019)

Identifiants

Citer

Ingo Bartholomäus, Laura Milan-Lobo, Annette Nicke, Sébastien Dutertre, Hanne Hastrup, et al.. Glycine transporter dimers: evidence for occurrence in the plasma membrane.. Journal of Biological Chemistry, 2008, 283 (16), pp.10978-10991. ⟨10.1074/jbc.M800622200⟩. ⟨hal-02306874⟩
82 Consultations
83 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More