Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF Signaling, Suggesting that Their Interacting Adipokine Role is Unique to Mammals
Résumé
In mammals, leptin and tumor-necrosis factor (TNF) are prominent interacting adipokines
mediating appetite control and insulin sensitivity. While TNF pleiotropically functions in immune
defense and cell survival, leptin is largely confined to signaling energy stores in adipocytes. Knowledge
about the function of avian leptin and TNF is limited and they are absent or lowly expressed in
adipose, respectively. Employing radiation-hybrid mapping and FISH-TSA, we mapped TNF and
its syntenic genes to chicken chromosome 16 within the major histocompatibility complex (MHC)
region. This mapping position suggests that avian TNF has a role in regulating immune response.
To test its possible interaction with leptin within the immune system and beyond, we compared
the transcription patterns of TNF, leptin and their cognate receptors obtained by meta-analysis of
GenBank RNA-seq data. While expression of leptin and its receptor (LEPR) were detected in the
brain and digestive tract, TNF and its receptor mRNAs were primarily found in viral-infected and
LPS-treated leukocytes. We confirmed leptin expression in the duodenum by immunohistochemistry
staining. Altogether, we suggest that whereas leptin and TNF interact as adipokines in mammals,
in birds, they have distinct roles. Thus, the interaction between leptin and TNF may be unique
to mammals.
Domaines
Autre [q-bio.OT]
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2019_Seroussi_International_Journal_Molecular_Sciences.pdf (1.88 Mo)
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