Article Dans Une Revue Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology Année : 2007

Identification of cDNAs encoding HSP70 and HSP90 in the abalone Haliotis tuberculata: Transcriptional induction in response to thermal stress in hemocyte primary culture

Résumé

Heat-shock proteins are a multigene family of proteins whose expression is induced by a variety of stress factors. This work reports the cloning and sequencing of HSP70 and HSP90 cDNAs in the gastropod Haliotis tuberculata. The deduced amino acid sequences of both HSP70 and HSP90 from H. tuberculata shared a high degree of homology with their homologues in other species, including typical eukaryotic HSP70 and HSP90 signature sequences. We examined their transcription expression pattern in abalone hemocytes exposed to thermal stress. Real-time PCR analysis indicated that both HSP70 and HSP90 mRNA were expressed in control animals but rapidly increased after heat-shock.

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Dates et versions

hal-02278095 , version 1 (04-09-2019)

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Emilie Farcy, Antoine Serpentini, Bruno Fiévet, Jean-Marc Lebel. Identification of cDNAs encoding HSP70 and HSP90 in the abalone Haliotis tuberculata: Transcriptional induction in response to thermal stress in hemocyte primary culture. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 2007, 146 (4), pp.540-550. ⟨10.1016/j.cbpb.2006.12.006⟩. ⟨hal-02278095⟩
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