Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2017

Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion

Résumé

During tumor progression, cancer cells acquire the ability to escape the primary tumor and invade adjacent tissues. They migrate through the stroma to reach blood or lymphatics vessels that will allow them to disseminate throughout the body and form metastasis at distant organs. To assay invasion capacity of cells in vitro, multicellular spheroids of cancer cells, mimicking primary tumor, are commonly embedded in collagen I extracellular matrix, which mimics the stroma. However, due to their higher density, spheroids tend to sink at the bottom of the collagen droplets, resulting in the spreading of the cells on two dimensions. We developed an innovative method based on droplet microfluidics to embed and control the position of multicellular spheroids inside spherical droplets of collagen. In this method cancer cells are exposed to a uniform three-dimensional (3D) collagen environment resulting in 3D cell invasion.
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Dates et versions

hal-02273775 , version 1 (29-08-2019)

Identifiants

  • HAL Id : hal-02273775 , version 1

Citer

Fabien Bertillot, Youmna Attieh, Morgan Delarue, Basile Gurchenkov, Stephanie Descroix, et al.. Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion. 3D cell culture, pp.269-279, 2017. ⟨hal-02273775⟩
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