Bioprocess often associates bioreactor and membrane separation. Bioprocess performances are intrinsically related to the capacity to control and to maintain microbial cells to their optimal activity (integrity, viability and productivity) while industrial operating conditions generate stressful environment or at least no ideal conditions. In present case, high shear rates and Joule effects are generated within a specific dynamic filtration module. Then, Residence Time Distribution (RTD) in a module, named Rotating and Vibrating Filtration (RVF), in which a three-blade impeller rotates between two membranes, was studied in laminar (Remixing<1×103) and turbulent (Remixing>3×104) regimes. In addition, thermal effects of mixing rate under several flow rates and regimes were reported. The magnitude and duration of stressful conditions stand as critical parameters which were quantified and modeled to engineer and to design intensive bioprocess.