Stimuli-responsive membrane activity of cyclic-peptide–polymer conjugates
Matthias Hartlieb
(1, 2)
,
Sylvain Catrouillet
(3, 2)
,
Agnès Kuroki
(2)
,
Carlos Sanchez-Cano
(2)
,
Raoul Peltier
(2)
,
Sébastien Perrier
(2, 4, 5)
1
Department of Chemistry [University of Warwick]
2 University of Warwick [Coventry]
3 ICGM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
4 Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, Parkville
5 Warwick Medical School
2 University of Warwick [Coventry]
3 ICGM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
4 Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, Parkville
5 Warwick Medical School
Sylvain Catrouillet
- Fonction : Auteur
- PersonId : 20480
- IdHAL : sylvain-catrouillet
- ORCID : 0000-0002-5132-6129
- IdRef : 184442648
Sébastien Perrier
- Fonction : Auteur
- PersonId : 16999
- IdHAL : sebastien-perrier
Résumé
Cyclic peptide nanotubes (CPNT) consisting of an even number of amino acids with an alternating chirality are highly interesting materials in a biomedical context due to their ability to insert themselves into cellular membranes. However, unwanted unspecific interactions between CPNT and non-targeted cell membranes are a major drawback. To solve this issue we have synthetized a series of CPNT–polymer conjugates with a cleavable covalent connection between macromolecule and peptide. As a result, the polymers form a stabilizing and shielding shell around the nanotube that can be cleaved on demand to generate membrane active CPNT from non-active conjugates. This approach enables us to control the stacking and lateral aggregation of these materials, thus leading to stimuli responsive membrane activity. Moreover, upon activation, the systems can be adjusted to form nanotubes with an increased length instead of aggregates. We were able to study the dynamics of these systems in detail and prove the concept of stimuli responsive membrane interaction using CPNT–polymer conjugates to permeabilize liposomes as well as mammalian cell membranes.
Domaines
ChimieFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Stimuli-responsive membrane activity of cyclic-peptide–polymer conjugates
|
Résumé |
en
Cyclic peptide nanotubes (CPNT) consisting of an even number of amino acids with an alternating chirality are highly interesting materials in a biomedical context due to their ability to insert themselves into cellular membranes. However, unwanted unspecific interactions between CPNT and non-targeted cell membranes are a major drawback. To solve this issue we have synthetized a series of CPNT–polymer conjugates with a cleavable covalent connection between macromolecule and peptide. As a result, the polymers form a stabilizing and shielding shell around the nanotube that can be cleaved on demand to generate membrane active CPNT from non-active conjugates. This approach enables us to control the stacking and lateral aggregation of these materials, thus leading to stimuli responsive membrane activity. Moreover, upon activation, the systems can be adjusted to form nanotubes with an increased length instead of aggregates. We were able to study the dynamics of these systems in detail and prove the concept of stimuli responsive membrane interaction using CPNT–polymer conjugates to permeabilize liposomes as well as mammalian cell membranes.
|
Auteur(s) |
Matthias Hartlieb
1, 2
, Sylvain Catrouillet
3, 2
, Agnès Kuroki
2
, Carlos Sanchez-Cano
2
, Raoul Peltier
2
, Sébastien Perrier
2, 4, 5
1
Department of Chemistry [University of Warwick]
( 93079 )
- University of Warwick, Coventry CV4 7AL, UK
- Royaume-Uni
2
University of Warwick [Coventry]
( 302824 )
- Coventry CV4 7AL
- Royaume-Uni
3
ICGM -
Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
( 1087107 )
- Place Eugène Bataillon - Bâtiment 17 - CC1700- 34095 Montpellier cedex 5
- France
4
Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, Parkville
( 435436 )
- Australie
5
Warwick Medical School
( 493176 )
- University of Warwick
Coventry CV4 7AL
United Kingdom
- Royaume-Uni
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2019-05-29
|
Date de publication électronique |
2019-04-18
|
Volume |
10
|
Numéro |
21
|
Page/Identifiant |
5476-5483
|
Domaine(s) |
|
Financement |
|
Projet(s) Européen(s) |
|
DOI | 10.1039/c9sc00756c |
Origine :
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