Article Dans Une Revue Journal of the American College of Cardiology Année : 2017

Fibrogenic Potential of PW1/Peg3 Expressing Cardiac Stem Cells

Olivier Claude
  • Fonction : Auteur
Guillaume Lebreton
Catherine Blanc
David Sassoon
Sébastien Hulot
  • Fonction : Auteur

Résumé

Background: Pw1 gene expression is a marker of adult stem cells in a wide range of tissues. PW1-expressing cells are detected in the heart but are not well characterized. Objectives: The authors characterized cardiac PW1-expressing cells and their cell fate potentials in normal hearts and during cardiac remodeling following myocardial infarction (MI). Methods: A human cardiac sample was obtained from a patient presenting with reduced left ventricular (LV) function following a recent MI. The authors used the PW1nLacZ+/- reporter mouse to identify, track, isolate, and characterize PW1-expressing cells in the LV myocardium in normal and ischemic conditions 7 days after complete ligature of the left anterior descending coronary artery. Results: In both human and mouse ischemic hearts, PW1 expression was found in cells that were mainly located in the infarct and border zones. Isolated cardiac resident PW1+ cells form colonies and have the potential to differentiate into multiple cardiac and mesenchymal lineages, with preferential differentiation into fibroblast-like cells but not into cardiomyocytes. Lineage-tracing experiments revealed that PW1+ cells differentiated into fibroblasts post-MI. Although the expression of c-Kit and PW1 showed little overlap in normal hearts, a marked increase in cells coexpressing both markers was observed in ischemic hearts (0.1 ± 0.0% in control vs. 5.7 ± 1.2% in MI; p < 0.001). In contrast to the small proportion of c-Kit+/PW1- cells that showed cardiogenic potential, c-Kit+/PW1+ cells were fibrogenic. Conclusions: This study demonstrated the existence of a novel population of resident adult cardiac stem cells expressing PW1+ and their involvement in fibrotic remodeling after MI.

Dates et versions

hal-02267419 , version 1 (19-08-2019)

Identifiants

Citer

Elisa Yaniz-Galende, Maguelonne Roux, Sophie Nadaud, Nathalie Mougenot, Marion Bouvet, et al.. Fibrogenic Potential of PW1/Peg3 Expressing Cardiac Stem Cells. Journal of the American College of Cardiology, 2017, 70 (6), pp.728-741. ⟨10.1016/j.jacc.2017.06.010⟩. ⟨hal-02267419⟩
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