Flagellin-mediated activation of IL-33-ST2 signaling by a pathobiont promotes intestinal fibrosis - Archive ouverte HAL
Article Dans Une Revue Mucosal Immunology Année : 2019

Flagellin-mediated activation of IL-33-ST2 signaling by a pathobiont promotes intestinal fibrosis

Jin Imai
  • Fonction : Auteur
Sho Kitamoto
  • Fonction : Auteur
Kohei Sugihara
  • Fonction : Auteur
Hiroko Kitamoto
  • Fonction : Auteur
Atsushi Hayashi
  • Fonction : Auteur
Tina Morhardt
  • Fonction : Auteur
Peter Kuffa
  • Fonction : Auteur
Nobuhiko Kamada
  • Fonction : Auteur

Résumé

Intestinal fibrosis is a severe complication in patients with Crohn's disease (CD). Unfortunately, the trigger leading to the development of intestinal fibrosis in the context of CD remains elusive. Here, we show that colonization by a CD-associated pathobiont adherent-invasive Escherichia coli (AIEC) promotes the development of intestinal fibrosis. Exogenously inoculated AIEC strain LF82 and commensal E. coli HS were gradually eradicated from the intestine in healthy mice. In Salmonella-or dextran sodium sulfate-induced colitis models, AIEC exploited inflammation and stably colonize the gut. Consequently, persistent colonization by AIEC LF82 led to substantial fibrosis. In contrast, commensal E. coli HS was unable to derive a growth advantage from inflammation, thereby failing to colonize the inflamed intestine or promote intestinal fibrosis. AIEC colonization potentiated the expression of the IL-33 receptor ST2 in the intestinal epithelium, which is crucial for the development of intestinal fibrosis. The induction of ST2 by AIEC LF82 was mediated by flagellin, as the Delta fliC mutant failed to induce ST2. These observations provide novel insights into pathobiont-driven intestinal fibrosis and can lead to the development of novel therapeutic approaches for the treatment of intestinal fibrosis in the context of CD that target AIEC and/or its downstream IL-33-ST2 signaling.

Dates et versions

hal-02193572 , version 1 (24-07-2019)

Identifiants

Citer

Jin Imai, Sho Kitamoto, Kohei Sugihara, Hiroko Kitamoto, Atsushi Hayashi, et al.. Flagellin-mediated activation of IL-33-ST2 signaling by a pathobiont promotes intestinal fibrosis. Mucosal Immunology, 2019, 12 (3), pp.632-643. ⟨10.1038/s41385-019-0138-4⟩. ⟨hal-02193572⟩
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