Comparative analysis of cellular and tissular expression of c-fos in human keratinocytes: evidence of its role in cell differentiation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Oncogene Année : 1994

Comparative analysis of cellular and tissular expression of c-fos in human keratinocytes: evidence of its role in cell differentiation

J. P. Moles
  • Fonction : Auteur
A. Tesnieres
  • Fonction : Auteur
C. Gauthier-Rouviere
  • Fonction : Auteur
S. Richard
  • Fonction : Auteur
J. J. Guilhou
  • Fonction : Auteur

Résumé

Recent studies on normal and pathological skin have suggested a role of the c-fos proto-oncogene in keratinocyte differentiation. To further elucidate this question we have used keratinocyte and skin culture models to study in vitro regulation of c-fos expression and attempted to correlate it with the keratinocyte maturation process. Our results show that c-fos expression is prolonged in keratinocyte monolayers both at the mRNA and protein level. Extracellular calcium which stimulate keratinocyte differentiation is able to induce c-fos expression in the presence of growth factors. However this c-fos expression cannot be maintained by these factors as seen in normal human skin in vivo. Conversely, spontaneous expression of c-fos can be seen in reconstituted skin when the neo-epidermis has completed its differentiation. All these data strongly support a role of c-fos as a switch between the early and late phases of keratinocyte differentiation allowing them to be definitively committed to their elimination process. Additionally, a differential regulation of c-fos seems to exist between keratinocyte culture and reconstituted epidermis, suggesting that tissular and serum factors are involved in the prolonged c-fos expression observed in human epidermis.
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Dates et versions

hal-02193238 , version 1 (24-07-2019)

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  • HAL Id : hal-02193238 , version 1
  • PUBMED : 8108118

Citer

N. Basset-Seguin, P. Demoly, J. P. Moles, A. Tesnieres, C. Gauthier-Rouviere, et al.. Comparative analysis of cellular and tissular expression of c-fos in human keratinocytes: evidence of its role in cell differentiation. Oncogene, 1994, 9 (3), pp.765--71. ⟨hal-02193238⟩
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