SUMO2/3 modification of cyclin E contributes to the control of replication origin firing - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2013

SUMO2/3 modification of cyclin E contributes to the control of replication origin firing

Résumé

The small ubiquitin-like modifier (SUMO) pathway is essential for the maintenance of genome stability. We investigated its possible involvement in the control of DNA replication during S phase by using the Xenopus cell-free system. Here we show that the SUMO pathway is critical to limit the number and, thus, the density of replication origins that are activated in early S phase. We identified cyclin E, which regulates cyclin-dependent kinase 2 (Cdk2) to trigger origin firing, as an S-phase substrate of this pathway. We show that cyclin E is dynamically and highly conjugated to SUMO2/3 on chromatin, independently of Cdk2 activity and origin activation. Moreover, cyclin E is the predominant SUMO2/3 target on chromatin in early S phase, as cyclin E depletion abolishes, while its readdition restores, the SUMO2/3 signal. Together, our data indicate that cyclin E SUMOylation is important for controlling origin firing once the cyclin E-Cdk2 complex is recruited onto replication origins.
Fichier principal
Vignette du fichier
ncomms2875.pdf (975.25 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02191601 , version 1 (10-06-2021)

Licence

Paternité

Identifiants

Citer

C. Bonne-Andrea, M. Kahli, F. Mechali, J. M. Lemaitre, G. Bossis, et al.. SUMO2/3 modification of cyclin E contributes to the control of replication origin firing. Nature Communications, 2013, 4, pp.1850. ⟨10.1038/ncomms2875⟩. ⟨hal-02191601⟩
29 Consultations
69 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More