Article Dans Une Revue Environmental Science and Pollution Research Année : 2017

Assessment of alternatives to environmental toxic formalin for DNA conservation in biological specimens

Emeline Sarot
  • Fonction : Auteur
Francis Duranthon
  • Fonction : Auteur
Stephane Pyronnet
  • Fonction : Auteur

Résumé

One essential step of museum and clinical specimen preservation is immersion in a fixative fluid to prevent degradation. Formalin is the most largely used fixative, but its benefit is balanced with its toxic and carcinogenic status. Moreover, because formalin-fixation impairs nucleic acids recovery and quality, current museum wet collections and formalin- fixed, paraffin-embedded clinical samples do not represent optimal tanks of molecular information. Our study has been developed to compare formalin to two alternative fixatives (RCL2® and ethanol) in a context of molecular exploitation. Based on a unique protocol, we created mammalian fixed collections, simulated the impact of time on preservation using an artificial ageing treatment and followed the evolution of specimens’ DNA quality. DNA extraction yield, purity, visual integrity and qualitative and quantitative ability to amplify the Cox1 gene were assessed. Our results show that both RCL2 and ethanol exhibit better performances than formalin. They do not impair DNA extraction yield, and more importantly, DNA alteration is delayed over the preservation step. The use of RCL2 or ethanol as fixative in biological collections may insure a better exploitation of the genetic resources they propose.

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Dates et versions

hal-02185460 , version 1 (17-07-2019)

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Citer

Emeline Sarot, Marie-Françoise Carillo-Baraglioli, Francis Duranthon, Amandine Péquignot, Stephane Pyronnet. Assessment of alternatives to environmental toxic formalin for DNA conservation in biological specimens. Environmental Science and Pollution Research, 2017, 24 (20), pp.16985-16993. ⟨10.1007/s11356-017-9349-y⟩. ⟨hal-02185460⟩
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