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Article Dans Une Revue F1000Research Année : 2016

Combining combing and secondary ion mass spectrometry to study DNA on chips using 13C and 15N labeling

Aaron Bensimon
  • Fonction : Auteur

Résumé

Dynamic secondary ion mass spectrometry ( D-SIMS) imaging of combed DNA - the combing, imaging by SIMS or CIS method - has been developed previously using a standard NanoSIMS 50 to reveal, on the 50 nm scale, individual DNA fibers labeled with different, non-radioactive isotopes in vivo and to quantify these isotopes. This makes CIS especially suitable for determining the times, places and rates of DNA synthesis as well as the detection of the fine-scale re-arrangements of DNA and of molecules associated with combed DNA fibers. Here, we show how CIS may be extended to (13)C-labeling via the detection and quantification of the (13)C (14)N (-) recombinant ion and the use of the (13)C: (12)C ratio, we discuss how CIS might permit three successive labels, and we suggest ideas that might be explored using CIS.
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Dates et versions

hal-02180963 , version 1 (01-10-2019)

Identifiants

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Armelle Cabin-Flaman, Anne-Francoise Monnier, Yannick Coffinier, Jean-Nicolas Audinot, David Gibouin, et al.. Combining combing and secondary ion mass spectrometry to study DNA on chips using 13C and 15N labeling. F1000Research, 2016, 5, pp.1437. ⟨10.12688/f1000research.8361.1⟩. ⟨hal-02180963⟩
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