Influence of Aptamer Surface Coverage on Small Target Recognition: A SPR and QCM-D Comparative Study
Résumé
Aptamers have emerged as promising biorecognition elements for the development of biosensors. The present work focused on the direct detection, by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D), of a low molecular weight (LMW) compound (less than 200 Da) with an aptamer receptor presented as an oriented monolayer on surface. These techniques are powerful for label-free, real-time characterization and quantification of molecular interactions at interfaces. Herein, we analyzed the influence of aptamer surface density on the recognition properties. A decrease of the surface
Domaines
Chimie théorique et/ou physiqueOrigine | Fichiers produits par l'(les) auteur(s) |
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