The cytochrome bc1 complex inhibitor Ametoctradin has an unusual binding mode - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochimica biophysica acta (BBA) - Bioenergetics Année : 2018

The cytochrome bc1 complex inhibitor Ametoctradin has an unusual binding mode

Résumé

Ametoctradin is an agricultural fungicide that selectively inhibits the cytochrome bc1 complex of oomycetes. Previous spectrophotometric studies using the purified cytochrome bc1 complex from Pythium sp. showed that Ametoctradin binds to the Qo-site of the enzyme. However, as modeling studies suggested a binding mode like that of the substrate ubiquinol, the possibility for a dual Qo- and Qi-site binding mode was left open. In this work, binding studies and enzyme assays with mitochondrial membrane preparations from Pythium sp. and an S. cerevisiae strain with a modified Qi-site were used to investigate further the binding mode of Ametoctradin. The results obtained argue that the compound could bind to both the Qo- and Qi-sites of the cytochrome bc1 complex and that its position or binding pose in the Qi-site differs from that of Cyazofamid and Amisulbrom, the two Qi-site-targeting, anti-oomycetes compounds. Furthermore, the data support the argument that Ametoctradin prefers binding to the reduced cytochrome bc1 complex. Thus, Ametoctradin has an unusual binding mode and further studies with this compound may offer the opportunity to better understand the catalytic cycle of the cytochrome bc1 complex.

Dates et versions

hal-02177613 , version 1 (09-07-2019)

Identifiants

Citer

Adalbert Dreinert, Antje Wolf, Tobias Mentzel, Brigitte Meunier, Marcus Fehr. The cytochrome bc1 complex inhibitor Ametoctradin has an unusual binding mode. Biochimica biophysica acta (BBA) - Bioenergetics, 2018, 1859 (8), pp.567--576. ⟨10.1016/j.bbabio.2018.04.008⟩. ⟨hal-02177613⟩
86 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More