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Article Dans Une Revue BIOMOLECULAR NMR ASSIGNMENTS Année : 2018

H-1, C-13 and N-15 backbone resonance assignment of the lamin C-terminal region specific to prelamin A

Résumé

Lamins are the main components of the nucleoskeleton. They form a protein meshwork that underlies the inner nuclear membrane. Mutations in the LMNA gene coding for A-type lamins (lamins A and C) cause a large panel of human diseases, referred to as laminopathies. These diseases include muscular dystrophies, lipodystrophies and premature aging diseases. Lamin A exhibits a C-terminal region that is different from lamin C and is post-translationally modified. It is produced as prelamin A and it is then farnesylated, cleaved, carboxymethylated and cleaved again in order to become mature lamin A. In patients with the severe Hutchinson-Gilford progeria syndrome, a specific single point mutation in LMNA leads to an aberrant splicing of the LMNA gene preventing the post-translational processing of prelamin A. This leads to the accumulation of a permanently farnesylated lamin A mutant lacking 50 amino acids named progerin. We here report the NMR H-1, N-15, (CO)-C-13, C-13 and C-13 chemical shift assignment of the C-terminal region that is specific to prelamin A, from amino acid 567 to amino acid 664. We also report the NMR H-1, N-15, (CO)-C-13, C-13 and C-13 chemical shift assignment of the C-terminal region of the progerin variant, from amino acid 567 to amino acid 614. Analysis of these chemical shift data confirms that both prelamin A and progerin C-terminal domains are largely disordered and identifies a common partially populated -helix from amino acid 576 to amino acid 585. This helix is well conserved from fishes to mammals.
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Dates et versions

hal-02177006 , version 1 (08-07-2019)

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Florian Celli, Ambre Petitalot, Camille Samson, François-Xavier Theillet, Sophie Zinn-Justin. H-1, C-13 and N-15 backbone resonance assignment of the lamin C-terminal region specific to prelamin A. BIOMOLECULAR NMR ASSIGNMENTS, 2018, 12 (2), pp.225--229. ⟨10.1007/s12104-018-9813-8⟩. ⟨hal-02177006⟩
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