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Journal Articles Journal of Applied Crystallography Year : 2019

Protein crystal structure determination with the crystallophore, a nucleating and phasing agent

Abstract

Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches.

Dates and versions

hal-02176249 , version 1 (08-07-2019)

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Sylvain Engilberge, Tristan Wagner, Gianluca Santoni, Cécile Breyton, Seigo Shima, et al.. Protein crystal structure determination with the crystallophore, a nucleating and phasing agent. Journal of Applied Crystallography, 2019, 52 (4), ⟨10.1107/S1600576719006381⟩. ⟨hal-02176249⟩
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