Bio-based porous zinc phosphates material with silver nanoparticles prepared from casein protein by hydrothermal synthesis for applications in biological environments - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2016

Bio-based porous zinc phosphates material with silver nanoparticles prepared from casein protein by hydrothermal synthesis for applications in biological environments

Jessica Hinostroza Ramos
  • Fonction : Auteur
Lydie Ploux

Résumé

The development of silver-based materials has attracted great interest from researchers principally for biomedical and pharmaceutical applications due to the antimicrobial properties and low human toxicity of silver ions in comparison to other heavy metals. Silver nanoparticles supported on inorganic structures such as zeolites, zinc oxide, phosphates, etc., present advantages compared to organic compounds due to their stability and safety 2. Porous zeolite/Ag and fibre-glass/Ag for example are used in water purification to control microbiological contamination. Thus, porous zinc phosphates material containing silver is a promising candidate for this application. Here we report a new approach to substitute inorganic reagents by raw materials with natural origin for the synthesis of zinc phosphates and silver nanoparticles by a hydrothermal method. Hopeite (Zn3(PO4)2.4H2O) enriched in casein and Ag nanoparticles (named Hopeite/CN/Ag°) was prepared by hydrothermal synthesis from the mixture of casein (CN), zinc (II) and silver (I) nitrate solutions. Casein protein from bovine milk was used as a bio-source of phosphorus for the zinc phosphates synthesis as well as a stabilizer in the preparation of silver nanoparticles. Ag nanoparticles with diameter size around 16.0 nm were in situ formed and incorporated in hopeite during the synthesis process. After calcination of the as-synthetized sample, another zinc phosphate material (α-Zn3(PO4)2 and α-Zn2P2O7) enriched in Ag nanoparticles (named ZnPOs/Ag°) was obtained. The antibacterial activity and cytotoxicity of these two materials containing Ag nanoparticles were evaluated. The morphology, porosity and structure of samples were also investigated. Adhesion tests of E. coli SCC1 (Fig. 1) on surfaces after different exposure times to bacteria show that the both solids are not favorable to bacterial growth when compared to a positive control (silicon wafer (WS)). Furthermore, among all the samples, ZnPOs/Ag° reveals a biocide effect after 3h of exposure to bacteria which is ascribed to the released Ag + ions. Moreover, biocompatibility of sample ZnPOs/Ag° for bone marrow stromal cells (STRO-1) was also observed. These results show that sample ZnPOs/Ag° is an interesting antimicrobial and biocompatible material for water disinfection by filtration and biomaterials applications respectively.

Domaines

Matériaux
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Dates et versions

hal-02174141 , version 1 (04-07-2019)

Identifiants

  • HAL Id : hal-02174141 , version 1

Citer

Jessica Hinostroza Ramos, Karine Anselme, Angelique Simon-Masseron, Lydie Ploux. Bio-based porous zinc phosphates material with silver nanoparticles prepared from casein protein by hydrothermal synthesis for applications in biological environments. Matériaux 2018, Sep 2016, Darmstad, Germany. ⟨hal-02174141⟩
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