Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Plant Biology Année : 2019

Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions

Résumé

Background - Dry pea production has increased substantially in North America over the last few decades. With this expansion, significant yield losses have been attributed to an escalation in Fusarium root rots in pea fields. Among the most significant rot rotting pathogenic fungal species, Fusarium solani fsp. pisi (Fsp) is one of the main causal agents of root rot of pea. High levels of partial resistance to Fsp has been identified in plant genetic resources. Genetic resistance offers one of the best solutions to control this root rotting fungus. A recombinant inbred population segregating for high levels of partial resistance, previously single nucleotide polymorphism (SNP) genotyped using genotyping-by-sequencing, was phenotyped for disease reaction in replicated and repeated greenhouse trials. Composite interval mapping was deployed to identify resistance-associated quantitative trait loci (QTL). Results - Three QTL were identified using three disease reaction criteria: root disease severity, ratios of diseased vs. healthy shoot heights and dry plant weights under controlled conditions using pure cultures of Fusarium solani fsp. pisi. One QTL Fsp-Ps 2.1 explains 44.4-53.4% of the variance with a narrow confidence interval of 1.2 cM. The second and third QTL Fsp-Ps3.2 and Fsp-Ps3.3 are closely linked and explain only 3.6-4.6% of the variance. All of the alleles are contributed by the resistant parent PI 180693. Conclusion - With the confirmation of Fsp-Ps 2.1 now in two RIL populations, SNPs associated with this region make a good target for marker-assisted selection in pea breeding programs to obtain high levels of partial resistance to Fusarium root rot caused by Fusarium solani fsp. pisi.
Fichier principal
Vignette du fichier
2019_BMC Plant Biology_Coyne_1.pdf (703.15 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02171437 , version 1 (27-05-2020)

Licence

Paternité

Identifiants

Citer

Clarice J Coyne, Lyndon D Porter, Gilles Boutet, Yu Ma, Rebecca J Mcgee, et al.. Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions. BMC Plant Biology, 2019, 19 (1), pp.98. ⟨10.1186/s12870-019-1699-9⟩. ⟨hal-02171437⟩
47 Consultations
32 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More