A Proteomic View of Cellular Responses to Anticancer Quinoline-Copper Complexes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proteomes Année : 2019

A Proteomic View of Cellular Responses to Anticancer Quinoline-Copper Complexes

Résumé

Metal-containing drugs have long been used in anticancer therapies. The mechansims of action of platinum-based drugs are now well-understood, which cannot be said of drugs containing other metals, such as gold or copper. To gain further insights into such mechanisms, we used a classical proteomic approach based on two-dimensional elelctrophoresis to investigate the mechanisms of action of a hydroxyquinoline-copper complex, which shows promising anticancer activities, using the leukemic cell line RAW264.7 as the biological target. Pathway analysis of the modulated proteins highlighted changes in the ubiquitin/proteasome pathway, the mitochondrion, the cell adhesion-cytoskeleton pathway, and carbon metabolism or oxido-reduction. In line with these prteomic-derived hypotheses, targeted validation experiments showed that the hydroxyquinoline-copper complex induces a massive reduction in free glutathione and a strong alteration in the actin cytoskeleton, suggesting a multi-target action of the hydroxyquinoline-copper complex on cancer cells.
Fichier principal
Vignette du fichier
proteomes-07-00026 (1).pdf (5.13 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02171140 , version 1 (02-07-2019)

Licence

Paternité

Identifiants

Citer

Bastien Dalzon, Joanna Bons, Hélène Diemer, Véronique Collin-Faure, Caroline Marie-Desvergne, et al.. A Proteomic View of Cellular Responses to Anticancer Quinoline-Copper Complexes. Proteomes, 2019, 7 (2), pp.E26. ⟨10.3390/proteomes7020026⟩. ⟨hal-02171140⟩
78 Consultations
18 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More