As nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in Nanomedicine have been concerned. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" nanoparticles, the in vitro an d in vivo NPs' behaviors.. SuperParamagnetic Iron Oxide Nanoparticles (SPIONs) is one of the most prominent agents because of their superparamagnetic properties, useful for separation applications. To mimic surface properties of different type NPs, core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) (positive, neutral and negative), SiO2 (positive and negative), titanium dioxide and metal gold.. The different surface SPION were incubated at a fixed serum:nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica surfaces where proteins preferred binding the neutral and positively charged. The importance of surface material on protein adsorption was also revealed by the preferential binding on TiO2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas.