Neutralization of cholera toxin by rat IgA secretory antibodies induced by a free synthetic peptide. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 1989

Neutralization of cholera toxin by rat IgA secretory antibodies induced by a free synthetic peptide.

A. Delmas
A Gruaz-Guyon
  • Fonction : Auteur
P Pedoussaut
  • Fonction : Auteur
P. Pierre
  • Fonction : Auteur
P Rivaille
  • Fonction : Auteur
J Vaerman
  • Fonction : Auteur

Résumé

Secretory immunoglobulin A (sIgA) is the major immunoglobulin in the bile of several species. They contribute to local immune defences of the gut. The protection against cholera toxin (CT) is due to the presence of specific sIgA in the bile and in the gut. We have already reported that oral administration of the peptide corresponding to the sequence 50-75 of cholera toxin B subunit elicits serum antibodies neutralizing CT activity, and that IgA and local protection are observed in the intestine of P50-75 orally immunized mice. In this study, we demonstrate the potential of this synthetic peptide as immunogen without carrier or adjuvant, not only in a strain known to be sensitive to CT, but also in an outbred one. Furthermore, this peptide stimulates the mucosal immunity, since we show that P50-75 induced-sIgA purified from rats bile and serum, are capable of neutralizing CT activity in the in vivo intestinal ligated loop test.
Fichier non déposé

Dates et versions

hal-02146949 , version 1 (04-06-2019)

Identifiants

  • HAL Id : hal-02146949 , version 1
  • PUBMED : 2930538

Citer

A. Delmas, A Gruaz-Guyon, P Pedoussaut, P. Pierre, P Rivaille, et al.. Neutralization of cholera toxin by rat IgA secretory antibodies induced by a free synthetic peptide.. Biochemical and Biophysical Research Communications, 1989, 159 (2), pp.707-12. ⟨hal-02146949⟩
20 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More