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Article Dans Une Revue Methods in Molecular Biology Année : 2012

A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections

Résumé

Real-time imaging of fungal infections is becoming integral to the study of host-pathogen interactions, as it allows monitoring of the spatial and temporal progression of pathogen growth or of the host response in a single animal as well as reducing the number of animals used to obtain signifi cant data. We present different applications of a novel luciferase reporter gene constructed from the coding sequences of the Candida albicans PGA59 gene, encoding a GPI-linked cell wall protein, and the Gaussia princeps luciferase gene. Upon addition of the coelenterazine substrate, light produced by the surface-exposed luciferase can be used to quantify gene expression from a variety of C. albicans promoters as well as monitoring cutaneous, subcutaneous, and vaginal infections.
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hal-02146745 , version 1 (07-06-2019)

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Donatella Pietrella, Brice Enjalbert, Ute S Zeidler, Sadri Znaidi, Anna Rachini, et al.. A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections. Methods in Molecular Biology, 2012, Host-Fungus Interactions. Methods and Protocols, 845, pp.537-546. ⟨10.1007/978-1-61779-539-8_39⟩. ⟨hal-02146745⟩
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