Mitogen-activated protein kinase (MAP kinase) activation inXenopus oocytes: Roles of MPF and protein synthesis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular Reproduction and Development Année : 1993

Mitogen-activated protein kinase (MAP kinase) activation inXenopus oocytes: Roles of MPF and protein synthesis

Résumé

Mitogen-activated protein kinase (MAP kinase) is a serine/threonine kinase whose enzymatic activity is thought to play a crucial role in mitogenic signal transduction and also in the progesterone-induced meiotic maturation of Xenopus oocytes. We have purified MAP kinase from Xenopus oocytes and have shown that the protein is present in metaphase II oocytes under two different forms: an inactive 41-kD protein able to autoactivate and to autophosphorylate in vitro, and an active 42-kD kinase resolved into two tyrosine phosphorylated isoforms on 2D gels. During meiotic maturation, MAP kinase becomes tyrosine phosphorylated and activated following the activation of the M-phase promoting factor (MPF), a complex between the p34cdc2 kinase and cyclin B. In vivo, MAP kinase activity displays a different stability in metaphase I and in metaphase II: protein synthesis is required to maintain MAP kinase activity in metaphase I but not in metaphase II oocytes. Injection of either MPF or cyclin B into prophase oocytes promotes tyrosine phosphorylation of MAP kinase, indicating that its activation is a downstream event of MPF activation. In contrast, injection of okadaic acid, which induces in vivo MPF activation, promotes only a very weak tyrosine phosphorylation of MAP kinase, suggesting that effectors other than MPF are required for the MAP kinase activation. Moreover, in the absence of protein synthesis, cyclin B and MPF are unable to promote in vivo activation of MAP kinase, indicating that this activation requires the synthesis of new protein(s).

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hal-02144066 , version 1 (29-05-2019)

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Olivier Haccard, Catherine Jessus, Hélène Rime, Jozef Goris, Wilfried Merlevede, et al.. Mitogen-activated protein kinase (MAP kinase) activation inXenopus oocytes: Roles of MPF and protein synthesis. Molecular Reproduction and Development, 1993, 36 (1), pp.96-105. ⟨10.1002/mrd.1080360114⟩. ⟨hal-02144066⟩
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