Characterization of a mycobacterial cellulase and its impact on biofilm- and drug-induced cellulose production - Archive ouverte HAL Access content directly
Journal Articles Glycobiology Year : 2017

Characterization of a mycobacterial cellulase and its impact on biofilm- and drug-induced cellulose production

Abstract

It was recently shown that Mycobacterium tuberculosis produces cellulose which forms an integral part of its extracellular polymeric substances within a biofilm set-up. Using Mycobacterium smegmatis as a proxy model organism, we demonstrate that M. smegmatis biofilms treated with purified MSMEG\₆752 releases the main cellulose degradation-product (cellobiose), detected by using ionic chromatography, suggesting that MSMEG\₆752 encodes a cellulase. Its overexpression in M. smegmatis prevents spontaneous biofilm formation. Moreover, the method reported here allowed detecting cellobiose when M. smegmatis cultures were exposed to a subinhibitory dose of rifampicin. Overall, this study highlights the role of the MSMEG\₆752 in managing cellulose production induced during biofilm formation and antibiotic stress response.

Dates and versions

hal-02137580 , version 1 (23-05-2019)

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Niël van Wyk, David Navarro, Mickaël Blaise, Jean-Guy Berrin, Bernard Henrissat, et al.. Characterization of a mycobacterial cellulase and its impact on biofilm- and drug-induced cellulose production. Glycobiology, 2017, 27 (5), pp.392-399. ⟨10.1093/glycob/cwx014⟩. ⟨hal-02137580⟩
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