HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor - Archive ouverte HAL Access content directly
Journal Articles Chemical Engineering Science Year : 2017

HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

Abstract

This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.
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Dates and versions

hal-02134833 , version 1 (20-05-2019)

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Rossmery A. Rodríguez-Hinestroza, Carmen López, Josep López-Santín, Cheikhou Kane, M. Dolors Benaiges, et al.. HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor. Chemical Engineering Science, 2017, 158, pp.196-207. ⟨10.1016/j.ces.2016.10.010⟩. ⟨hal-02134833⟩
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