Mice Carrying a Hypomorphic Evi1 Allele Are Embryonic Viable but Exhibit Severe Congenital Heart Defects - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2014

Mice Carrying a Hypomorphic Evi1 Allele Are Embryonic Viable but Exhibit Severe Congenital Heart Defects

Emi Bard-Chapeau
  • Function : Author
Bindya Jacob
  • Function : Author
Belinda Chua
  • Function : Author
Gouri Chatterjee
  • Function : Author
Jerrold Ward
  • Function : Author
Fatma Urun
  • Function : Author
Emi Kinameri
  • Function : Author
Sayadi Ahmed
  • Function : Author
Shoumo Bhattacharya
  • Function : Author
Motomi Osato
  • Function : Author
Archibald Perkins
  • Function : Author
Adrian Moore
  • Function : Author
Nancy Jenkins
  • Function : Author

Abstract

The ecotropic viral integration site 1 (Evi1) oncogenic transcription factor is one of a number of alternative transcripts encoded by the Mds1 and Evi1 complex locus (Mecom). Overexpression of Evi1 has been observed in a number of myeloid disorders and is associated with poor patient survival. It is also amplified and/or overexpressed in many epithelial cancers including nasopharyngeal carcinoma, ovarian carcinoma, ependymomas, and lung and colorectal cancers. Two murine knockout models have also demonstrated Evi1's critical role in the maintenance of hematopoietic stem cell renewal with its absence resulting in the death of mutant embryos due to hematopoietic failure. Here we characterize a novel mouse model (designated Evi1fl3) in which Evi1 exon 3, which carries the ATG start, is flanked by loxP sites. Unexpectedly, we found that germline deletion of exon3 produces a hypomorphic allele due to the use of an alternative ATG start site located in exon 4, resulting in a minor Evi1 N-terminal truncation and a block in expression of the Mds1-Evi1 fusion transcript. Evi1δex3/δex3 mutant embryos showed only a mild non-lethal hematopoietic phenotype and bone marrow failure was only observed in adult Vav-iCre/+, Evi1fl3/fl3 mice in which exon 3 was specifically deleted in the hematopoietic system. Evi1δex3/δex3 knockout pups are born in normal numbers but die during the perinatal period from congenital heart defects. Database searches identified 143 genes with similar mutant heart phenotypes as those observed in Evi1δex3/δex3 mutant pups. Interestingly, 42 of these congenital heart defect genes contain known Evi1-binding sites, and expression of 18 of these genes are also effected by Evi1 siRNA knockdown. These results show a potential functional involvement of Evi1 target genes in heart development and indicate that Evi1 is part of a transcriptional program that regulates cardiac development in addition to the development of blood.

Dates and versions

hal-02130275 , version 1 (15-05-2019)

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Emi Bard-Chapeau, Dorota Szumska, Bindya Jacob, Belinda Chua, Gouri Chatterjee, et al.. Mice Carrying a Hypomorphic Evi1 Allele Are Embryonic Viable but Exhibit Severe Congenital Heart Defects. PLoS ONE, 2014, 9 (2), pp.e89397. ⟨10.1371/journal.pone.0089397⟩. ⟨hal-02130275⟩
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