Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm. - Archive ouverte HAL Access content directly
Journal Articles Molecular Microbiology Year : 2001

Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm.

E. Bouveret
  • Function : Author
A. Rigal
  • Function : Author
R Lloubes
  • Function : Author
C Lazdunski
  • Function : Author

Abstract

Several proteins of the Tol/Pal system are required for group A colicin import into Escherichia coli. Colicin A interacts with TolA and TolB via distinct regions of its N-terminal domain. Both interactions are required for colicin translocation. Using in vivo and in vitro approaches, we show in this study that colicin A also interacts with a third component of the Tol/Pal system required for colicin import, TolR. This interaction is specific to colicins dependent on TolR for their translocation, strongly suggesting a direct involvement of the interaction in the colicin translocation step. TolR is anchored to the inner membrane by a single transmembrane segment and protrudes into the periplasm. The interaction involves part of the periplasmic domain of TolR and a small region of the colicin A N-terminal domain. This region and the other regions responsible for the interaction with TolA and TolB have been mapped precisely within the colicin A N-terminal domain and appear to be arranged linearly in the colicin sequence. Multiple contacts with periplasmic-exposed Tol proteins are therefore a general principle required for group A colicin translocation.
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Dates and versions

hal-02130124 , version 1 (15-05-2019)

Identifiers

  • HAL Id : hal-02130124 , version 1
  • PUBMED : 11703658

Cite

Laure Journet, E. Bouveret, A. Rigal, R Lloubes, C Lazdunski, et al.. Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm.. Molecular Microbiology, 2001, 42 (2), pp.331-44. ⟨hal-02130124⟩
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