Time resolved fluorescence properties of phenylalanine in different environments. Comparison with molecular dynamics simulation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biophysical Chemistry Année : 1998

Time resolved fluorescence properties of phenylalanine in different environments. Comparison with molecular dynamics simulation.

Norbert Garnier
G Cremel
  • Fonction : Auteur
G Nullans
  • Fonction : Auteur
P. Hubert
  • Fonction : Auteur
M. Vincent
  • Fonction : Auteur
J Gallay
  • Fonction : Auteur
M Genest
  • Fonction : Auteur

Résumé

Time resolved fluorescence of the phenylalanine residue (Phe) alone and included in the transmembrane domain (TMD) sequences of the epidermal growth factor receptor (EGFR) and ErbB-2 was studied using the synchrotron radiation source of light, and compared to molecular dynamics (MD) simulations. The fluorescence intensity decay is strongly sensitive to the environment. A mono-exponential decay was obtained for Phe amino acid alone in two different solvents and for Phe included in EGFR transmembrane sequence, with fluorescence lifetime values varying from 1.7 ns (EGFR) to 7.4 ns (Phe dissolved in water). In ErbB-2 transmembrane sequence three lifetimes were detected. The relative amplitude of the shortest one (0.14 ns) is smaller than 10%, whereas the others (0.6 and 2.2 ns) are almost equally represented. They have been attributed to different rotamers exchanging slowly. This interpretation is supported by MD simulations which evidence transitions in time series of the chi 1 dihedral angle of Phe observed in the case of ErbB-2. The anisotropy decays are similar for both peptides and indicate the presence of a correlation time in the nanosecond range (1-4 ns) and the probable existence of a very fast one (< 0.05 ns). Autocorrelation functions computed from MD simulations corroborate these results.
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hal-02123796 , version 1 (09-05-2019)

Identifiants

  • HAL Id : hal-02123796 , version 1
  • PUBMED : 9697301

Citer

J-P Duneau, Norbert Garnier, G Cremel, G Nullans, P. Hubert, et al.. Time resolved fluorescence properties of phenylalanine in different environments. Comparison with molecular dynamics simulation.. Biophysical Chemistry, 1998, 73 (1-2), pp.109-19. ⟨hal-02123796⟩
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