Chapitre D'ouvrage Année : 2019

Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation

Résumé

Ultrasound-mediated gene delivery is an interesting approach, which could help in increasing gene transfer in deep tissues. Moreover, it allows for performing experiments guided by the image to determine which elements are required. Microbubbles complexed with a eukaryotic expression cassette are excellent agents as they are responsive to ultrasounds and, upon oscillation, can destabilize membranes to enhance gene transfer. Here, we describe the preparation of positively charged microbubbles, plasmid free of antibiotic resistance marker, their combination and the conditions of ultrasound-mediated liver transfection post-systemic administration in mice. This association allowed us to obtain a superior liver gene expression at least over 8 months after a single injection.

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Dates et versions

hal-02123117 , version 1 (07-05-2019)

Identifiants

Citer

Nathalie Mignet, Corinne Marie, Anthony Delalande, Simona Manta, Johanne Seguin, et al.. Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation. Nanotechnology for Nucleic Acid Delivery, pp.377-387, 2019, Methods in Molecular Biology, ⟨10.1007/978-1-4939-9092-4_25⟩. ⟨hal-02123117⟩
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