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Article Dans Une Revue Journal of Magnetic Resonance Année : 1999

Determination of and Coupling Constants in 13C-Labeled Nucleic Acids Using Constant-Time HMQC

A Hu
  • Fonction : Auteur
Eugene Skripkin
  • Fonction : Auteur
Abdelali Kettani
  • Fonction : Auteur
Weidong Hu
  • Fonction : Auteur

Résumé

A novel 1H-13C correlated two-dimensional experiment, CT-HMQC-J, for the measurement of three-bond proton-phosphorus coupling constants in 13C-labeled DNA is described. The experiment is based on the intensity difference of 1H-13C cross peaks in the presence and absence of the proton-phosphorus coupling interaction during the constant-time period in HMQC experiment. The 3J(H, P) coupling constants can be easily extracted from the intensity ratios of the two experiments. The method has been applied to a uniformly 13C, 15N-labeled d(GGAGGAT) 7-mer DNA sample. The proton-phosphorus coupling constants determined from CT-HMQC-J, together with the other three-bond coupling constants, are used to determine beta and epsilon torsion angles. The introduction of beta and epsilon restraints has improved the convergence as well as the quality of d(GGAGGAT) structure.

Dates et versions

hal-02122551 , version 1 (07-05-2019)

Identifiants

Citer

A Hu, Serge Bouaziz, Eugene Skripkin, Abdelali Kettani, Weidong Hu. Determination of and Coupling Constants in 13C-Labeled Nucleic Acids Using Constant-Time HMQC. Journal of Magnetic Resonance, 1999, 139 (1), pp.181-185. ⟨10.1006/jmre.1999.1785⟩. ⟨hal-02122551⟩
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