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Article Dans Une Revue European Journal of Biochemistry Année : 1988

Studies of a novel repetitive sequence family in the genome of mice.

Martine Decoville
B. Malfoy
  • Fonction : Auteur

Résumé

A new middle repetitive sequence is described in the mouse genome. It has been revealed with a recombinant clone isolated from a Mus musculus BamHI gene library constructed in pBR322 and containing an insertion of 1.73 kb. When digests of genomic DNA were subjected to Southern blot hybridization, using the 1.73-kb insert as probe, we obtained a light smear and discrete bands, indicating a dispersion in the mouse genome of this sequence. This 1.73-kb sequence seems to be a part of a greater repetitive sequence at least 6 kb in length. The sizes of the bands hybridizing with the 1.73-kb insert are similar when compared between different laboratory strains but differ remarkably between the two species M. musculus and Mus caroli. We have shown also a great variation in the copy number of the sequence studied between these two species. When rat DNA is probed with the 1.73-kb insert, no hybridization is observed. Subcloning of the 1.73-kb sequence in three fragments has pointed out that the reiteration was not homogeneous along the 1.73-kb sequence. The 1.73-kb clone was sequenced and compared with other interspersed repetitive sequences, previously described in the rodent genome, and no homology was found.
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Dates et versions

hal-02119359 , version 1 (03-05-2019)

Identifiants

  • HAL Id : hal-02119359 , version 1
  • PUBMED : 2839338

Citer

Martine Decoville, B. Malfoy, D. Locker. Studies of a novel repetitive sequence family in the genome of mice.. European Journal of Biochemistry, 1988, 174 (4), pp.679-84. ⟨hal-02119359⟩
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