Article Dans Une Revue Developmental Biology Année : 2018

Loss of Tbx3 in murine neural crest reduces enteric glia and causes cleft palate, but does not influence heart development or bowel transit

Silvia Huerta López
  • Fonction : Auteur
Marina Avetisyan
  • Fonction : Auteur
Christina M Wright
  • Fonction : Auteur
Anne M Moon
  • Fonction : Auteur
Robert O Heuckeroth
  • Fonction : Auteur
  • PersonId : 1046529

Résumé

Transcription factors that coordinate migration, differentiation or proliferation of enteric nervous system (ENS) precursors are not well defined. To identify novel transcriptional regulators of ENS development, we performed microarray analysis at embryonic day (E) 17.5 and identified many genes that were enriched in the ENS compared to other bowel cells. We decided to investigate the T-box transcription factor Tbx3, which is prominently expressed in developing and mature ENS. Haploinsufficiency for TBX3 causes ulnar-mammary syndrome (UMS) in humans, a multi-organ system disorder. TBX3 also regulates several genes known to be important for ENS development. To test the hypothesis that Tbx3 is important for ENS development or function, we inactivated Tbx3 in all neural crest derivatives, including ENS progenitors using Wnt1-Cre and a floxed Tbx3 allele. Tbx3 fl/fl; Wnt1-Cre conditional mutant mice die shortly after birth with cleft palate and difficulty feeding. The ENS of mutants was well-organized with a normal density of enteric neurons and nerve fiber bundles, but small bowel glial cell density was reduced. Despite this,

Fichier principal
Vignette du fichier
Loss of Tbx3.pdf (2.04 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence
Loading...

Dates et versions

hal-02117793 , version 1 (02-05-2019)
hal-02117793 , version 2 (13-02-2020)

Licence

Identifiants

Citer

Silvia Huerta López, Marina Avetisyan, Christina M Wright, Karim Mesbah, Robert G. Kelly, et al.. Loss of Tbx3 in murine neural crest reduces enteric glia and causes cleft palate, but does not influence heart development or bowel transit. Developmental Biology, 2018, 444, pp.S337-S351. ⟨10.1016/j.ydbio.2018.09.017⟩. ⟨hal-02117793v2⟩
119 Consultations
302 Téléchargements

Altmetric

Partager

  • More