A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography - Archive ouverte HAL Access content directly
Journal Articles International Union of Crystallography journal Year : 2019

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Eric Girard
Bernard Lorber
Claude Sauter

Abstract

Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.
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Dates and versions

hal-02117153 , version 1 (22-01-2020)

Licence

Attribution - CC BY 4.0

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Raphaël de Wijn, Oliver Hennig, Jennifer Roche, Sylvain Engilberge, Kevin Rollet, et al.. A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography. International Union of Crystallography journal, 2019, 6 (3), pp.454-464. ⟨10.1107/S2052252519003622⟩. ⟨hal-02117153⟩
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