Testing for kavain in human hair using gas chromatography-tandem mass spectrometry. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences Année : 2003

Testing for kavain in human hair using gas chromatography-tandem mass spectrometry.

Résumé

A sensitive, specific and reproducible method for the quantitative determination of kavain in human hair has been developed. The sample preparation involved a decontamination step of the hair with methylene chloride. The hair sample (about 50 mg) was incubated in 1 ml of methanol for 1 h, in an ultrasonic bath, in presence of 20 ng of methaqualone-d7 used as internal standard. The methanolic solution was evaporated to dryness, and the residue reconstituted by adding 30 microl of methanol. A 2 microl aliquot of the extract was injected onto the column (Optima5-MS capillary column, 5% phenyl-95% methylsiloxane, 30 m x 0.25 mm i.d. x 0.25 mm film thickness) of a Hewlett-Packard (Palo Alto, CA) gas chromatograph (5890). Kavain was detected by its parent ion at m/z 230 and daughter ions at m/z 111 and 202 through a Finnigan TSQ 700 MS/MS system. The assay was capable of detecting 30 pg/mg of kavain (limit of detection (LOD)). Linearity was observed for kavain concentrations ranging from 100 to 2000 pg/mg with a correlation coefficient of 0.998. Intra-day precision at 400 pg/mg was 13.7%. The analysis of a segment of hair, obtained from an occasional consumer, revealed the presence of kavain at the concentration of 418 pg/mg. A higher concentration (1708 pg/mg) was detected in the corresponding pubic hair.

Domaines

Toxicologie
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Dates et versions

hal-02112828 , version 1 (27-04-2019)

Identifiants

  • HAL Id : hal-02112828 , version 1
  • PUBMED : 14643516

Citer

F. Villain, V Cirimele, A. Tracqui, Francois-Xavier Ricaut, B. Ludes, et al.. Testing for kavain in human hair using gas chromatography-tandem mass spectrometry.. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2003, 798 (2), pp.351-4. ⟨hal-02112828⟩
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