Zero-Mode Waveguide Detection of Flow-Driven DNA Translocation through Nanopores
Résumé
We directly measure the flow-driven injection of DNA through nanopores at the level of single molecule and single pore using a modified zero-mode waveguide method. We observe a flow threshold independent of the pore radius, the DNA concentration, and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed in the de Gennes-Brochard suction model. Finally, we show that the height of the energy barrier is modulated by functionalizing the nanopores.
Mots clés
nanopore
procedures
structure activity relation
Bacteriophage lambda
Benzoxazoles
Viral
Flow Injection Analysis
Fluorescent Dyes
Intercalating Agents
Models
Chemical
Quinolinium Compounds
Structure-Activity Relationship
Thermodynamics
Energy barriers
Nanopores
Waveguides
DNA concentration
DNA translocation
Flow injection
Pore radius
Single molecule
Zero-mode waveguides
benzoxazole derivative
fluorescent dye
intercalating agent
quinoline derivative
virus DNA
chemical model
chemistry
Enterobacteria phage lambda
genetics