Zero-Mode Waveguide Detection of Flow-Driven DNA Translocation through Nanopores
Résumé
We directly measure the flow-driven injection of DNA through nanopores at the level of single molecule and single pore using a modified zero-mode waveguide method. We observe a flow threshold independent of the pore radius, the DNA concentration, and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed in the de Gennes-Brochard suction model. Finally, we show that the height of the energy barrier is modulated by functionalizing the nanopores.
Mots clés
- genetics
- Waveguides
- DNA concentration
- DNA translocation
- Flow injection
- Pore radius
- Single molecule
- Zero-mode waveguides
- benzoxazole derivative
- fluorescent dye
- intercalating agent
- quinoline derivative
- virus DNA
- chemical model
- chemistry
- Enterobacteria phage lambda
- Fluorescent Dyes
- nanopore
- procedures
- structure activity relation
- Bacteriophage lambda
- Benzoxazoles
- Viral
- Flow Injection Analysis
- Nanopores
- Intercalating Agents
- Models
- Chemical
- Quinolinium Compounds
- Structure-Activity Relationship
- Thermodynamics
- Energy barriers