The Toxoplasma gondii inhibitor-2 regulates protein phosphatase 1 activity through multiple motifs. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Parasitology Research Année : 2017

The Toxoplasma gondii inhibitor-2 regulates protein phosphatase 1 activity through multiple motifs.

Résumé

Toxoplasma gondii has a complex life cycle characterized by multiple differentiation steps that are essential for its survival in both human and definitive feline host. Several studies have demonstrated the importance of phosphorylations by protein kinases during the life cycle of T. gondii. However, very little is known about protein phosphatases and their regulators in the parasite. We report the molecular and functional characterization of the T. gondii ortholog of the inhibitor-2 protein, designated TgI2. We show that TgI2 encompasses conserved motifs involved in the interaction and modulation of the phosphatase activity of T. gondii protein phosphatase 1, named TgPP1. We show that a specific combination of motifs is involved in binding and/or inhibition of the TgPP1 activity. We show here that the TgI2 protein is a potent inhibitor of TgPP1 phosphatase activity. TgI2 SILK and RVxF motifs are critical for regulating the activity of TgPP1, a feature that is common with the higher eukaryotes inhibitor-2 protein.

Domaines

Parasitologie
Fichier principal
Vignette du fichier
final I2 revised 2.pdf (1.05 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02106427 , version 1 (23-04-2019)

Identifiants

Citer

Quentin Deveuve, Kevin Lesage, Thomas Mouveaux, Mathieu Gissot. The Toxoplasma gondii inhibitor-2 regulates protein phosphatase 1 activity through multiple motifs.. Parasitology Research, 2017, 116 (9), pp.2417-2426. ⟨10.1007/s00436-017-5543-6⟩. ⟨hal-02106427⟩
50 Consultations
86 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More